Lipoprotein Protocols
  • Author : Jose M. Ordovas
  • Release Date : 10 November 2010
  • Publisher : Humana Press
  • Genre : Science
  • Pages : 284
  • ISBN 13 : 1617370312

Lipoprotein Protocols Book Summary

The development of new methodologies has played a key role in the advancement of all areas of research. Specifically, the initial advances in our understanding of lipoprotein structure and metabolism were made possible by the development of ultracentrifugation and electrophoretic techniques. More recently, the advent of molecular biological techniques opened possibilities that were unthinkable just a few decades ago. The use of the analytical ult- centrifuge to study plasma lipoproteins began in the 1940s with the work of Mutzenbecher, McFarlene, Pedersen, Gofman, Lindgren, and Elliot. Another crucial step, during the 1950s, was the development of this tool as a prepa- tive technique by Havel, Eder, and Bragdon, among others. This technolo- cal progress allowed investigators to "dig" deeper into the structure of these complex macromolecules made of lipids and proteins, and permitted inves- gators to continue unraveling the physical and chemical characteristics of the proteins associated with lipoprotein particles (apolipoproteins) and the enzymes involved in their processing. This information led to both a better understanding of the biological functions of the lipoprotein fractions and their constituents, and creation of a more comprehensive overall scheme for plasma lipoprotein metabolism. Several gaps in this puzzle were filled through the work of Goldstein and Brown, who elucidated the structure and role of the low-density lipoprotein - ceptor. This was the first identified among a profusion of receptors that are key for the cellular catabolism of these particles.

Lipoprotein Protocols

Lipoprotein Protocols

Author : Jose M. Ordovas
Publisher : Humana Press
Genre : Science
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File Size : 42,7 Mb
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The development of new methodologies has played a key role in the advancement of all areas of research. Specifically, the initial advances in our understanding of lipoprotein structure and metabolism were made possible by the development of ultracentrifugation and electrophoretic techniques. More recently, the advent of molecular biological techniques opened ...

Handbook of Lipoprotein Testing

Handbook of Lipoprotein Testing

Author : Nader Rifai,G. Russell Warnick,Marek H. Dominiczak
Publisher : Amer. Assoc. for Clinical Chemistry
Genre : Medical
Total View : 9197 Views
File Size : 53,8 Mb
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Flavoprotein Protocols

Flavoprotein Protocols

Author : Steven K. Chapman,Graeme A. Reid
Publisher : Springer Science & Business Media
Genre : Science
Total View : 4939 Views
File Size : 45,6 Mb
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As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be ...

2 D Proteome Analysis Protocols

2 D Proteome Analysis Protocols

Author : Andrew J. Link
Publisher : Springer Science & Business Media
Genre : Science
Total View : 6394 Views
File Size : 49,9 Mb
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With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a ...

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NMDA Receptor Protocols

Author : Min Li
Publisher : Springer Science & Business Media
Genre : Medical
Total View : 4067 Views
File Size : 53,8 Mb
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Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the ...

T Cell Protocols

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Author : Kelly P. Kearse
Publisher : Springer Science & Business Media
Genre : Medical
Total View : 2032 Views
File Size : 49,5 Mb
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The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology. Invariably, in putting together a book ...

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Adhesion Protein Protocols

Author : Elisabetta Dejana,Monica Corada
Publisher : Springer Science & Business Media
Genre : Science
Total View : 2850 Views
File Size : 45,9 Mb
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Adhesion molecules are of fundamental importance in the regulation of immunity, inflammation, tissue remodeling, and embryonic development. They comprise different families of homologous proteins, such as selectins, integrins, cadherins, and immunoglobins. In addition, beyond these groups, other str- tures with adhesive properties, such as proteoglycans, occludin, and CD44, have been ...

Cytochrome P450 Protocols

Cytochrome P450 Protocols

Author : Ian R. Phillips,Elizabeth A. Shephard
Publisher : Springer Science & Business Media
Genre : Science
Total View : 7248 Views
File Size : 49,5 Mb
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In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of ...